Gel permeation chromatography is a type of liquid chromatography that uses a polymer solution to pass through a column filled with gel, and separates by molecular size in the column.

The column is a glass column or a metal column, filled with spherical gel with a high degree of cross-linking. The spherical gel itself has many holes of different sizes according to a certain distribution. The polymer molecules are separated step by step, the macromolecules are separated first, and the smaller polymer molecules are repelled by the solvent molecules and then separated, and then a certain method is used to detect the concentration and molecular weight of the solute in each stage.

Gel chromatography (GPC) can measure the average molecular weight, molecular weight distribution, molecular weight distribution width of high molecular polymers (including olefin polymers), oligomers, carbohydrates, proteins, etc., and analyze homopolymers and impurities in copolymers .

1. Sample status: it can be liquid, powder, block sample.

2. The sample is required to have good solubility in the designated mobile phase, and the sample volume is required to be about 20mg.

3. If the solubility of the sample is poor, it is required to dissolve well in advance (the concentration is required to be about 2-5mg/ml), or provide a reasonable dissolution method (if the method is too complicated, needs to consult the account manager in advance).

relative molecular weight

absolute molecular weight